| Most departmental laboratories and associated instrumentation are located in the
Medical Sciences I complex and adjacent Irvine Hall. The labs contain a wide variety of
equipment for research in molecular and cellular physiology and molecular biophysics.
Knowledgeable users can help investigators in the Department exploit this equipment for
their projects. Microscopy: fluorescence, ratiometric calcium imaging,
confocal. (Cahalan, Chandy, Hall labs)
Electrophysiology and cell microinjection: patch clamp, microelectrode
recording (oocyte) setups. (Cahalan,
Chandy, Hall labs)
Molecular biology: protein (HPLC, FRLC) and nucleic acid work
(electrophoresis, DNA sequencing, PCR, electroporation, gel photography); programs for
analyzing protein and DNA sequences and for molecular visualization. (most labs)
Mass spectrometry: quadrupole-orthogonal-time-of-flight tandem mass
spectrometer (QSTAR XL, Applied Biosystems/MDS Sciex) which can be coupled to both
electrospray (ESI) and MALDI ionization sources for protein and peptide analysis and their
posttranslational modifications using either 1-dimentional or 2-dimensional liquid
chromatography coupled with tandem mass spectrometry. (Huang lab)
Optical spectroscopy: Absorbance, fluorescence, step-scan FTIR, and
circular dichroism. (Longmuir, Vickery, White labs)
Physicochemical characterization: Differential scanning calorimetry,
isothermal titration calorimetry, static and dynamic light scattering, BiaCore surface
plasmon resonance. (Longmuir, Vickery, White labs)
Dark room: Automated film developer and print maker.
Library: The Department maintains the previous three to five years of
major journals in physiology, biophysics, biochemistry, molecular biology, immunology, and
cell biology, as well as journals of broad scientific interest, and reference books.
Computers provide free access to on-line publications.
Machine shop: The Department maintains a shop
with milling machine and lathe for hands-on fabrication of chambers
or other devices.
LINKED FACILITY DESCRIPTIONS
Optical Biology Core Resource: http://dbc.bio.uci.edu/signupDef.html
BioImaging Interest Group: http://big.bio.uci.edu/
DNA Core Facility: http://www.genome.uci.edu/core.htm
DNA Array Core Facility:
• http://www.genome.uci.edu/DNAcore/services.htm
Transgenic Mouse Facility: http://darwin.bio.uci.edu/%7Etjf/
Machine Shops:
• http://www.bio.uci.edu/machineshop/machine_shop.html
• http://www.physsci.uci.edu/research/
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