PH.D., Harvard University, 1963
Chair, Department of Physiology & Biophysics
University of California, Irvine
D-349 Medical Sciences I,
Irvine, CA 92697-4025
jklanyi@uci.edu
UCI Faculty Profile
Bacteriorhodopsin, Halorhodopsin, Light-Driven Ion Pumps.
Dr. Lanyi studies the physiology, biochemistry, and the molecular mechanisms of ion translocation across biological membranes. His work focuses on two light driven electrogenic ion pumps in the cytoplasmic membrane of Halobacterium halobium, bacteriorhodopsin (an outward directed proton pump) and halorhodopsin (an inward directed chloride pump). These blue-purple pigments are proteins in which retinal isomerization after photon absorption initiates molecular rearrangements and results in ion uptake on one side of the membrane, translocation, then release on the other side. In bacteriorhodopsin, the reversible loss of a proton during the photocycle is thought to be the central event in the transport cycle. Halorhodopsin remains protonated (and positively charged) during the photocycle.
Individual steps of the photocycles are followed by flash-spectroscopy with a gated optical multichannel analyser, which reveals the rise and decay of several intermediate species with red- and blue-shifted absorption spectra. The measured difference spectra are deconvoluted, using computer intensive grid-search, into component spectra. The relative weights of these spectra are the time-dependent concentrations of the intermediates, from which the kinetics are reconstructed. The kinetic models reveal the steps for internal transfers of proton and chloride, and their release and uptake at the membrane surfaces during transport. The temperature dependencies of the rate constants provide information on the thermodynamics of energy coupling between the photoexcited retinal chromophore and the proton gradient created.
The kinetic and thermodynamic models for ion translocation, and the spectroscopic measurements, provide the framework for Dr. Lanyi's studies on the mechanistic aspects of transport in the two bacterial rhodopsins. The roles of individual amino acid residues are explored with site-specific mutagenesis, using a newly developed shuttle vector based on a halobacterial plasmid. Altered genes are expressed in H. halobium, which produces the proteins in large quantities and in a wild-type-like two-dimensional membrane array suitable also for diffraction studies of structure. In both ion pumps the emphasis has been on 1) describing the path of the ions as they travel across these membrane-spanning proteins and on understanding the nature of the forces which determine the rate and direction of their movements; and 2) explaining how the ions are exchanged between residues at the protein surface and the aqueous phase.
UCI Faculty Profile: Janos K. Lanyi.
Lanyi Lab Web-Site
R. Needleman, M. Chang, B. Ni, G. Váró, J. Fornes, S.H. White and J.K. Lanyi. Properties of Asp-212-asn Bacteriorhodopsin Suggest that Asp-212 and Asp-85 both Participate in a Counterion and Proton Acceptor Complex Near the Schiff Base. J.Biol. Chem. 266:11478-11484, 1991.
G. Váró and J.K. Lanyi. Thermodynamics and Energy Coupling in the Bacteriorhodopsin Photocycle. Biochemistry 30:5016-5022, 1991.
G. Váró and J.K. Lanyi. Kinetic and Spectroscopic Evidence for an Irreversible Step between Deprotonation and Reprotonation of the Schiff Base in the Bacteriorhodopsin Photocycle. Biochemistry 30:5008-5015, 1991.
G. Váró and J.K. Lanyi. Effects of the Crystalline Structure of Purple Membrane on the Kinetics and Energetics of the Bacteriorhodopsin Photocycle. Biochemistry 30:7165-7171, 1991.
Y. Cao, G. Váró, M. Chang, B. Ni, R. Needleman and J.K. Lanyi. Water is Required for Proton Transfer from Aspartate-96 to the Bacteriorhodopsin Schiff base. Biochemistry 30:10972-10979, 1991.
J.K. Lanyi, J. Tittor, G. Váró, G. Krippahl and D. Oesterhelt. Influence of the Size and Protonation State of Acidic Residue 85 on the Absorption Spectrum and Photoreaction of the Bacteriorhodopsin Chromophore. Biochim.Biophys.Acta 1009:102-110, 1992.
J.K. Lanyi. Proton Transfer and Energy Coupling in the Bacteriorhodopsin Photocycle. J.Bioenerg.Biomembr. 24:169-179, 1992.
J.K. Lanyi. Reaction Cycle and Thermodynamics in Bacteriorhodopsin. Acta Physiol.Scand. 146:245-248, 1992.
A. Maeda, J. Sasaki, Y. Shichida, T. Yoshizawa, M. Chang, B. Ni, R. Needleman, and J.K. Lanyi. Structures of Aspartic Acid-96 in the L and N Intermediates of Bacteriorhodopsin: Analysis by Fourier Transform Infrared Spectroscopy. Biochemistry 31:4684-4690, 1992.
G. Váró, L. Zimányi, M. Chang, B. Ni, R. Needleman and J.K. Lanyi. A Residue Substitution near the -ionone Ring of the Retinal Affects the M substates of Bacteriorhodopsin. Biophys.J. 61:820-826, 1992.
L. Zimányi, G. Váró, M. Chang, B. Ni, R. Needleman and J.K. Lanyi. Pathways of Proton Release in the Bacteriorhodopsin Photocycle. Biochemistry 31:8535-8543, 1992.
L. Zimányi and J.K. Lanyi. The Two Consecutive M Substates in the Photocycle of Bacteriorhodopsin are Affected Specifically by the D85N and D96N Residue Replacements. Photochem.Photobiol. 56: 1049-1055, 1992.
J. Sasaki, Y. Shichida, J.K. Lanyi and A. Maeda. Protein Changes Associated with Reprotonation of the Schiff Base in the Photocycle of Asp96-asn Bacteriorhodopsin. The MN Intermediate with Unprotonated Schiff Base but N-like Protein Structure. J.Biol.Chem. 267:20782-20786, 1992.
S. Druckmann, N. Friedman, J.K. Lanyi, R. Needleman, M. Ottolenghi, and M. Sheves. The Back Photoreaction of the M Intermediate in the Photocycle of Bacteriorhodopsin: Mechanism and Evidence for two M Species. Photochem.Photobiol. 56: 1041-1047, 1992.
J.K. Lanyi. From Time-resolved Difference Spectra to Kinetics, Mechanism and Thermodynamics in the Bacteriorhodopsin Photocycle. In: MEMBRANE PROTEINS: STRUCTURES, INTERACTIONS AND MODELS. (A. Pullman, J.Jortner, and B. Pullman, eds.) pp. 85-96, Kluwer Academic Publishers, Dordrecht, Holland, 1992.
M. Kataoka, K. Mihara, H. Kamikubo, R. Needleman, J.K. Lanyi, and F. Tokunaga. Trimeric Mutant Bacteriorhodopsin, D85N, shows a Monophasic CD Spectrum. FEBS Lett. 333:111-113, 1993.
J.K. Lanyi. Ion Transport Rhodopsins (Bacteriorhodopsin and Halorhodopsin): Structure and Function. In: THE BIOCHEMISTRY OF ARCHAE (Ed. M. Kates and A.T. Matheson) pp. 189-207, Elsevier Science Publishers, Amsterdam, 1993.
L. Zimányi and J.K. Lanyi. Deriving the Intermediate Spectra and the Photocycle Kinetics from Time-resolved Difference Spectra of Bacteriorhodopsin: The Simpler case of the Recombinant D96N Protein. Biophys.J. 64:240-251, 1993.
Y.N. Zhang, M.A. El-Sayed, M.L. Bonet, J.K. Lanyi, M. Chang, B. Ni and R. Needleman. Effects of Genetic Replacements of Charged and H-bonding Residues on Ca2+ Binding to Deionized Bacteriorhodopsin. Proc.Natl.Acad.Sci.U.S.A. 90:1445-1449, 1993.
Y. Cao, G. Váró, A.L. Klinger, M.S. Braiman, R. Needleman and J.K. Lanyi. Proton Transfer from Asp-96 to the Bacteriorhodopsin Schiff base is Caused by Decrease of the pKa of Asp-96 which Follows a Protein Backbone Conformation Change. Biochemistry 32:1981-1990, 1993.
L.S. Brown, L. Bonet, R. Needleman and J.K. Lanyi. Estimated Acid Dissociation Constants of the Schiff Base, Asp-85, and Arg-82 during the Bacteriorhodopsin Photocycle. Biophys.J. 65:124-130, 1993.
J.K. Lanyi. Pathways of Proton Transfer in the Light-driven Pump Bacteriorhodopsin. Experientia 49:514-517, 1993.
S. Druckmann, M.P. Heyn, J.K. Lanyi, M. Ottolenghi and L. Zimányi. Thermal Equilibration Between the M and N Intermediates in the Photocycle of Bacteriorhodopsin. Biophys. J. 65:1231-1234, 1993.
L. Zimányi, Y. Cao, R. Needleman, M. Ottolenghi and J.K. Lanyi. Pathway of Proton Uptake in the Bacteriorhodopsin Photocycle. Biochemistry 32:7669-7678, 1993.
J.K. Lanyi. Proton Translocation Mechanism and Energetics in the Light-driven Pump Bacteriorhodopsin. Biochim.Biophys.Acta Bioenergetics Reviews 1183:241-261, 1993.
L.S. Brown, L. Zimányi, M. Ottolenghi, R. Needleman and J.K. Lanyi. Photoreaction of the N Intermediate of Bacteriorhodopsin and its Relationship to the Decay Kinetics of the M Intermediate. Biochemistry 32:7679-7685, 1993.
Y. Cao, L.S. Brown, R. Needleman, and J.K. Lanyi. Relationship of Proton Uptake on the Cytoplasmic Surface and the Reisomerization of Retinal in the Bacteriorhodopsin Photocycle: an Attempt to Understand the Complex Kinetics of the pH Changes and the N and O Intermediates. Biochemistry 32:10239-10248, 1993.
J.K. Lanyi. Bioenergetics and Transport in Extreme Halophiles. In: THE BIOLOGY OF HALOPHILIC BACTERIA (Eds. R.E. Vreeland and L.I. Hochstein), pp. 289-310, CRC Press, Boca Raton, 1993.
A. Maeda, J. Sasaki, Y. Yamazaki, R. Needleman and J.K. Lanyi. Interaction of Aspartate 85 with a Water Molecule and the Protonated Schiff base in the L Intermediate of Bacteriorhodopsin; a Fourier Transform Infrared Spectroscopic Study. Biochemistry 33: 1713-1717, 1994.
L. Song, M.A. El-Sayed and J.K. Lanyi. Protein Catalysis of the Subpicosecond Photoisomerization in the Primary Process of Bacteriorhodopsin Photosynthesis. Science 261:891-894, 1993.
L.S. Brown, Y. Yamazaki, A. Maeda, L. Sun, R. Needleman and J.K. Lanyi. The Proton Transfers in the Cytoplasmic Domain of Bacteriorhodopsin are Facilitated by a Cluster of Interacting Residues. J.Mol.Biol. 239:401-414, 1994.
J.K. Lanyi. Halorhodopsin: a Prokarotic Light-driven Active Chloride Transport System. In: ELECTROGENIC Cl- TRANSPORTERS IN BIOLOGICAL MEMBRANES (G.A. Gerencher, ed.). pp. 1 - 16, Springer Verlag, Berlin, 1994.
J. Sasaki, J. K. Lanyi, R. Needleman, T. Yoshizawa, and A. Maeda. Complete Identification of C = O Stretching Vibrational Bands of Protonated Aspartic Acid Residues in the Difference Infrared Spectra of M and N Intermediates versus Bacteriorhodopsin. Biochemistry 33:3178-3184, 1994.
N. Friedman, S. Druckman, J.K. Lanyi, R. Needleman, A. Lewis, M. Ottolenghi, and M. Sheves. The Retinal-protein Covalent Bond in Bacteriorhodopsin is not Required for Forming a Photochemically Active Pigment Analogous to the Wild Type. Biochemistry 33:1971-1976, 1994.
L. S. Brown, Y. Gat, M. Sheves, Y. Yamazaki, Y., A. Maeda, R. Needleman, and J.K. Lanyi. The Retinal Schiff Base - Counterion Complex of Bacteriorhodopsin: Changed Geometry during the Photocycle is a Cause of Proton Transfer to Aspartate 85. Biochemistry 33: 12001-12011, 1994.
M. Kataoka, H. Kamikubo, F. Tokunaga, L. S. Brown, Y. Yamazaki, A. Maeda, M. Sheves, R. Needleman, and J.K. Lanyi. Energy Coupling in an Ion Pump: the Reprotonation Switch of Bacteriorhodopsin. J.Mol.Biol. 243: 621-638, 1994.
J.K. Lanyi. The Photochemical Reaction Cycle of Bacteriorhodopsin. In: Advances in Chemistry 240: Molecular and Biomolecular Electronics (Ed. R.R. Birge), pp. 491-510, ACS Press, 1994.
J.K. Lanyi. Bacteriorhodopsin: a Paradigm for Proton Pumps? Biophys.Chem. 56: 143-151, 1995.
J.F. Nagle, L. Zimányi, and J.K. Lanyi. Testing BR Photocycle Kinetics. Biophys.J. 68: 1490-1499, 1995.
Y. Yamazaki, J. Sasaki, M. Hatanaka, H. Kandori, R. Needleman, T. Shinada, K. Yoshihara, L.S. Brown, J.K. Lanyi and A. Maeda. Interaction of Tryptophan-182 with the 9-Methyl Group of the Retinal in the L Intermediate of Bacteriorhodopsin. Biochemistry 34: 577-582, 1995.
Y. Cao, L.S. Brown, J. Sasaki, A. Maeda, R. Needleman and J.K. Lanyi. Relationship of Proton Release at the Extracellular Surface to Deprotonation of the Schiff Base in the Bacteriorhodopsin Photocycle. Biophys. J. 68: 1518-1530, 1995.
H. Kandori, Y. Yamazaki, J. Sasaki, R. Needleman, J.K. Lanyi, and A. Maeda. Water-mediated Proton Transfer in Proteins: An FTIR Study of Bacteriorhodopsin. J.Am. Chem.Soc. 117: 2118-2119, 1995.
G. Váró, L. Zimányi, X. Fan, L. Sun, R. Needleman, and J.K. Lanyi. The Photocycle of Halorhodopsin from Halobacterium salinarium. Biophys. J. 68: 2062-2072, 1995.
J.K. Lanyi. Purification of Halorhodopsin from Halobacterium salinarium. In: ARCHAE, A LABORATORY MANUAL (eds. F.T. Robb, K.R. Sowers, S. DasSarma, A.R. Place, H.J. Schreier, and E.M. Fleischmann), pp. 59-61, Cold Spring Harbor Lab. Press, Cold Spring Harbor, 1995.
Y. Yamazaki, M. Hatanaka, H. Kandori, J. Sasaki, W.F. Jan Karstens, J. Raap, J. Lugtenburg, M. Bizounok, J. Herzfeld, R. Needleman, J.K. Lanyi, and A. Maeda. Water Structural Changes at the Proton Uptake Site (the Thr46-Asp96 Domain) in the L intermediate of Bacteriorhodopsin. Biochemistry 34: 7088-7093, 1995.
J.K. Lanyi. Bacteriorhodopsin as a Model for Proton Pumps. Nature 375: 461-463, 1995.
S. L. Logunov, M.A. El-Sayed and J.K. Lanyi. Photoisomerization Quantum Yield and Apparent Energy Content of the K Intermediate in the Photocycles of Bacteriorhodopsin, its Mutants D85N, R82Q, D212N, and Deionized Blue Bacteriorhodopsin. J.Phys. Chem. 100: 2391-2398, 1996.
S.L. Logunov, M. El-Sayed and J.K. Lanyi. Effects of the Replacement of Neutral Amino Acid Residues in the Retinal Binding Cavity of Bacteriorhodopsin on the Dynamics of its Primary Processes. Biophys.J. 70: 2875-2881, 1996.
J. Sasaki, L.S. Brown, Y.-S. Chon, H. Kandori, A. Maeda, R. Needleman and J.K. Lanyi. Conversion of Bacteriorhodopsin into a Chloride Ion Pump. Science 269: 73-75, 1995.
L.S. Brown, G. Váró, M. Hatanaka, J. Sasaki, H. Kandori, A. Maeda, M. Sheves, R. Needleman and J.K. Lanyi. The Complex Extracellular Domain Regulates the Deprotonation and Reprotonation of the Retinal Schiff Base during the Bacteriorhodopsin Photocycle. Biochemistry 34: 12903-12911, 1995.
G. Váró, L.S. Brown, J. Sasaki, A. Maeda, R. Needleman and J.K. Lanyi. Light-driven Chloride Ion Transport by Halorhodopsin from Natronobacterium pharaonis. I. The Photochemical Cycle. Biochemistry 34: 14490-14499, 1995.
G. Váró, R. Needleman and J.K. Lanyi. Light-driven Chloride Ion Transport by Halorhodopsin from Natronobacterium pharaonis. II. Chloride Release and Uptake, Protein Conformation Change, and Thermodynamics. Biochemistry 34: 14500-14507, 1995.
G. Váró and J.K. Lanyi. Effects of Hydrostatic Pressure on the Kinetics Reveal a Volume Increase During the Bacteriorhodopsin Photocycle. Biochemistry 34: 12161-12169, 1995.
L.S. Brown, G. Váró, R. Needleman and J.K. Lanyi. Functional Significance of a Protein Conformation Change at the Cytoplasmic End of Helix F during the Bacteriorhodopsin Photocycle. Biophys.J. 69: 2103-2111, 1995.
S. Masuda, M. Nara, M. Tasumi, M.A. El-Sayed and J.K. Lanyi. Fourier Transform Infrared Spectroscopic Studies of the Effect of Ca2+ Binding on the States of Aspartic Acid Side Chains in Bacteriorhodopsin. J.Phys.Chem. 99: 7776-7781, 1995.
L.S. Brown, J. Sasaki, H. Kandori, A. Maeda, R. Needleman, and J.K. Lanyi. Glutamatic Acid 204 is the Terminal Proton Release Group at the Extracellular Surface of Bacteriorhodopsin J.Biol.Chem. 270: 27122-27126, 1995.
L. Song, D. Yang, M.A. El-Sayed and J.K. Lanyi. Retinal Isomer Composition of Some Bacteriorhodopsin Mutants under Light and Dark Adaptation Conditions. J.Phys. Chem. 99: 10052-10055, 1995.
G. Váró, R. Needleman and J.K. Lanyi. Protein Structural Change at the Cytoplasmic Surface as the Cause of Cooperativity in the Bacteriorhodopsin Photocycle. Biophys.J. 70: 461-467, 1996.
L.S. Brown and J.K. Lanyi. Determination of the Transiently Decreased pKa of the Retinal Schiff base During the Photocycle of Bacteriorhodopsin. Proc.Natl.Acad.Sci. U.S.A. 93: 1731-1734, 1996.
H. Kamikubo, M. Kataoka, G. Váró, T. Oka, F. Tokunaga, R. Needleman and J.K. Lanyi. Structure of the N Intermediate of Bacteriorhodopsin Revealed by X-ray Diffraction. Proc.Natl.Acad.Sci. U.S.A. 93: 1386-1390, 1996.
H.-T. Richter, L.S. Brown, R. Needleman and J.K. Lanyi. A Linkage of the pKa's of Asp-85 and Glu-204 Forms Part of the Reprotonation Switch of Bacteriorhodopsin. Biochemistry 35: 4054-4062, 1996.
Y. Yamazaki, S. Tuzi, H. Saitô, H. Kandori, R. Needleman, J.K. Lanyi and A. Maeda. Hydrogen-Bonds of Water and C = O Groups Coordinate Long-range Structural Changes in the L Photointermediate of Bacteriorhodopsin. Biochemistry 35: 4063-4068, 1996.
G. Váró, L.S. Brown, R. Needleman and J.K. Lanyi. Proton Transport by Halorhodopsin. Biochemistry 35: 6604-6611, 1996.
M. Hatanaka, J. Sasaki, H. Kandori, T.G. Ebrey, R. Needleman, J.K. Lanyi and A. Maeda. Effects of Arginine-82 on the Interactions of Internal Water Molecules in Bacteriorhodopsin. Biochemistry 35: 6308-6312, 1996.
S. Tuzi, S. Yamaguchi, A. Naito, R. Needleman, J.K. Lanyi and H. Saitô. Conformation and Dynamics of [3-13C]Ala-labeled Bacteriorhodopsin or Bacterio-opsin Induced by an Interaction with Retinal or Its Analogs as Studied by 13C NMR. Biochemistry 35: 7520-7527, 1996.
J.K. Lanyi and G. Váró. The Photocycles of Bacteriorhodopsin. Israel J. Chem. 35: 365-386, 1996.
O. Weidlich, B. Schalt, N. Friedmann, M. Sheves, J. K. Lanyi, L. S. Brown and F. Siebert. Steric Interaction Between the 9-Methyl Group of the Retinal and Tryptophan 182 Controls 13-cis to all-trans Reisomerization and Proton Uptake in the Bacteriorhodopsin Photocycle. Biochemistry 35: 10807-10814, 1996.
Y.-S. Chon, J. Sasaki, H. Kandori, L. S. Brown, J. K. Lanyi, R. Needleman and A. Maeda. Hydration of the Counterion of the Schiff Base in the Chloride Transporting Mutant of Bacteriorhodopsin: FTIR and FT-Raman Studies of the Effects of Anion Binding when Asp85 is Replaced with a Neutral Residue. Biochemistry 35: 14244-14250, 1996.
J.L. Spudich and J.K. Lanyi. Shuttling Between Two Protein Conformations: the Common Mechanism for Sensory Transduction and Ion Transport. Curr. Opinion Cell. Biol. 8: 452-457, 1996.
H.-T. Richter, R. Needleman, H. Kandori, A. Maeda and J.K. Lanyi. Relationship of Retinal Configuration and Internal Proton Transfer at the End of the Bacteriorhodopsin Photocycle. Biochemistry 35: 15461-15466, 1996.
H.-T. Richter, R. Needleman and J.K. Lanyi. Perturbed Interaction Between Residues 85 and 204in Tyr-185->Phe andAsp-85->Glu Bacteriorhodopsins. Biophys.J. 71: 3392-3398, 1996.
L.S. Brown, R. Needleman and J.K. Lanyi. Interaction of Proton and Chloride Transfer Pathways in Recombinant Bacteriorhodopsin with Chloride Transport Activity: Implications for the Chloride Translocation Mechanism. Biochemistry 35: 16048-16054, 1996.
L. Zimányi and J.K. Lanyi. Fourier Transform Raman Study of Retinal Isomeric Composition and Equilibration in Halorhodopsin. J.Phys.Chem. B, 101: 1930-1933, 1997.
J.K. Lanyi. Mechanism of Ion Transport Across Membranes: Bacteriorhodopsin as a Prototype for Proton Pumps. J.Biol.Chem. 272: 31209-31212, 1997.
H. Kandori, Y. Yamazaki, M. Hatanaka, R. Needleman, L.S. Brown, H.-T. Richter, J.K. Lanyi and A. Maeda. Time-Resolved FTIR Study of Structural Changes in the Last Steps of the Photocycles of Glu-204 and Leu-93 Mutants of Bacteriorhodopsin. Biochemistry 36: 5134-5141, 1997.
T.E. Thorgeirsson, W. Xiao, L.S. Brown, R. Needleman, J.K. Lanyi, and Y.-K. Shin. Transient Channel-Opening in Bacteriorhodopsin: An EPR Study. J.Mol.Biol. 273: 951-957, 1997.
M. Hatanaka, R. Kashima, H. Kandori, N. Friedman, M. Sheves, R. Needleman, J.K. Lanyi and A. Maeda. Trp-86 ® Phe Replacement in Bacteriorhodopsin Affects a Water Molecule near Asp85 and Light Adaptation. Biochemistry 36: 5493-5498, 1997.
A. Maeda, H. Kandori, Y. Yamazaki, S. Nishimura, M. Hatanaka, Y.-S. Chon, R. Needleman and J.K. Lanyi. Intramembrane Signaling Mediated by Hydrogen-Bonding of Water and Carboxyl Groups in Bacteriorhodopsin and Rhodopsin. J. Biochem. (Tokyo) 121: 399-406, 1997.
T. Oka, H. Kamikubo, F. Tokunaga, J.K. Lanyi, R. Needleman and M. Kataoka. X-ray Diffraction Studies of Bacteriorhodopsin. Determination of the Positions of Mercury Label at Several Engineered Cysteine Residues. Photochem.Photobiol. 66: 768-773, 1997.
H. Kamikubo, T. Oka, F. Tokunaga, Y. Imamoto, J.K. Lanyi, and M. Kataoka. The Last Phase of the Reprotonation Switch in Bacteriorhodopsin: The Transition between the M-type and the N-type Protein Conformation Depends on Water of Hydration. Biochemistry 36: 12282-12287, 1997.
R. Renthal, Y.-J. Chung, R. Escamilla, L.S. Brown and J.K. Lanyi. Guanidium Restores the Chromophore but not Rapid Proton Release in Bacteriorhodopsin Mutant R82Q. Biophys.J. 73: 2711-2717, 1997.
S. Hashimoto, K. Obata, H. Takeuchi, R. Needleman, and J.K. Lanyi. Ultraviolet Resonance Raman Spectra of Trp-182 and Trp-189 in Bacteriorhodopsin: Novel Information on the Structure of Trp-182 and its Steric Interaction with Retinal. Biochemistry 36: 11583-11590, 1997.
J.K. Lanyi and A. Maeda. Structural Basis of Information Transfer and Energy Transduction in Rhodopsins. Photochem.Photobiol. 66: 733-734, 1997.
A.K. Dioumaev, H.-T. Richter, L.S. Brown, M. Tanio, S. Tuzi, H. Saitô, Y. Kimura, R. Needleman and J.K. Lanyi. Existence of a Proton Transfer Chain in Bacteriorhodopsin: Participation of Glu-194 in the Release of Protons to the Extracellular Surface. Biochemistry 37: 2496-1506, 1998.
S. Yamaguchi, S. Tuzi, T. Seki, M. Tanio, R. Needleman, J.K. Lanyi, A. Naito and H. Saitô. Stability of the C-terminal a-helical Domain of Bacteriorhodopsin that Protrudes from the Membrane Surface, as Studied by High-resolution Solid-state 13C NMR. J. Biochem (Tokyo) 123: 78-86, 1998.
L.S. Brown, A.K. Dioumaev, R. Needleman and J.K. Lanyi. Local-Access Model for Proton Transfer in Bacteriorhodopsin. Biochemistry (in press).
J.K. Lanyi. The Local-Access Mechanism of Proton Transport by Bacteriorhodopsin. Biochem.Biophys.Acta Bioenergetics (in press).
List of Publications via PubMed (NIH National Library of Medicine)