Lectures
Chromatin Structure and Function
BC225: Winter 2008
11:00-12:20 pm Monday and 9:00-10:20 am Thursday
Rm 240D (Biochem Conference Room), Med Sci I
Will be held every other year.
Instructors:
Drs. Paolo Sassone-Corsi, Joan Stefan, Leslie M.
Thompson, Paul Vrana, Marcelo Wood, and Kyoko Yokomori
Course Description:
BC225 Course on Chromatin Structure and Function
Chromatin structural organization plays critical roles in
regulating genome functions and the maintenance of genome integrity. This
course focuses on current topics concerning chromatin structure and functions as
well as nuclear dynamics in eukaryotes. Topics include histone and DNA
modifications and modifying enzymes, chromatin assembly and remodeling,
chromatin-mediated transcriptional regulation, genomic imprinting, X chromosome
dosage compensation, siRNA-mediated gene silencing, mitotic chromosome
dynamics, chromosome territories and nuclear matrix, and chromatin regulation
in DNA repair. Finally, the
importance of chromatin regulation in human diseases will be discussed. In each session, there will be a short
lecture followed by student presentations of key research papers. This course
should be registered as "GR".
No exams. MB203 and 204 are
required prerequisites. Exception
can be made by permission of the instructors.
If there are any
questions, please contact Kyoko Yokomori (X48215, kyokomor@uci.edu).
Course Schedule
BC225 Chromatin Structure and Function, Winter 2006
Monday 11:00-12:20
Thursday 9:00-10:20
| Mon, 1/7/08 | (1) Introduction (Vrana/Thompson/Sassone-Corsi/Yokomori) | |
| Thu, 1/10/08 | (2) Chromatin Architecture Histone modifications (Thompson) Gilbert 2004 (pdf), Genes Dev 2006 (pdf), Nature 2002 (pdf), Nature 2007 (pdf) Lecture Notes, Kouzarides Cell 2007 |
|
| Mon, 1/14/08 | (3) Histone modifying enzymes (Sassone-Corsi) | |
| Thu, 1/17/08 | (4) ATP-dependent chromatin
remodeling (Steffan) Cairns EMBO 2008, Whitehouse Nature 2007, News & Views Whitehouse, Peterson NSMB 2007, Lessard Neuron 2007, Lessard Data, News & Reviews Lessard, Flaus EMBO 2004, Chromatin Remodeling, Nucleo Rem Fac, Lecture Notes, Matt Presentation |
|
| Mon, 1/21/08 | Holiday | |
| Thu, 1/24/08 | (5) Chromatin - Transcriptional
initiation and elongation Chromatin and DNA replication (Steffan) Smale Genes and Dev 2006, Ross EMBO 2006, Allis Genes and Dev 2007, Allis suppl (doc), Allis news and views, Harikrishnan Nature Gen 2005, Cell Workman Role of Chromatin, Groth cell DNA repair, Lecture Notes |
|
| Mon, 1/28/08 | (6) Epigenetic regulation of
transcription Chromatin changes in germ lines (Sassone-Corsi) GovinJCB07, HyllusGD07, IwaseCell07, KadaNat07, KirmizisNat07, KrishnamoorthyGD06, PerilloSci08, TannyGD07 |
|
| Thu, 1/31/08 | (7) DNA methylation, genomic
imprinting/X chrom. inactivation (Vrana) Christine - Sakatani et al 2001, Maria - Shen et al 2007, Anwesh - Wood et al 2007, Rich - Science 2007, Lecture Notes |
|
| Mon, 2/4/08 | (8) Epigenetics, development & stem
cells (Vrana) Harry paper, Rosy paper, Nate paper, Anwash paper, Lecture Notes |
|
| Thu, 2/7/08 | (9) Environmental effects on
chromatin/epigenetics (Vrana) Lydia paper, Will paper, Harry paper, Rani paper, Christine paper |
|
| Mon, 2/11/08 | (10) Chromatin & epigenetics in
non-mammalian systems (Vrana) Kim's PDF, Will's PDF, Wan-Ting's PDF, Jiantao's PDF, Harry's PDF |
|
| Thu, 2/14/08 | (11) The role of chromatin modifying
enzymes in synaptic plasticity and memory formation (Wood) Maria-Miller and Sweatt, Nate-Guan et al, Nestler review 2007, Renthal et al, Rosy-Vecsey et al, Ruth-Fischer et al, Sweatt review 2007 |
|
| Mon, 2/18/08 | Holiday | |
| Thu, 2/21/08 | (12) RNAi in regulation of gene
expression, heterochromatin and applications to research
(Thompson) Exportin5 2003, Heterochromatin 2008, Multiplex RNAi 2008, miRNA and ployglutamine Bonini, p53 microRNA 2007, Cancer siRNA 2008, Hermeking Cell NV 2007, Wan-Ting Chen, Hsiang Ho, siRNA Thompson, Christine Marciniak, multiplex RNAi Science |
|
| Mon, 2/25/08 | (13) Higher order chromatin
structures and nuclear organization I Chromosome territory, centromeres and telomeres, heterochromatin and euchromatin, nuclear matrix (Yokomori) Bickmore rev 07, DevCell06Misteli, CB06Belmont, PNAS07Bickmore, NCB07SATB1, Lecture Notes |
|
| Thu, 2/28/08 | (14) Higher order chromatin
structures and nuclear organization II Sister chromatid cohesion and mitotic chromosome condensation (Yokomori) NRG03Meyer, PNAS07conII, EMBO07cohesinSA1, NasmythDevCell08, Nat08Peters, Lecture Notes |
|
| Mon, 3/3/08 | (15) Higher order chromatin structure
in gene regulation Role of LCR in the formation of active chromatin hub, interchromosomal interactions, Position effect variegation and insulator function (Yokomoi) MolCell08GATA, Cell06Axel, NatGen06deLaat, NatGen06Zhao, Lecture Notes |
|
| Thu, 3/6/08 | (16) Role of chromatin in DNA repair
(Yokomori) Chromatin remodeler rev, Sci07Koshland, Cell07LukasUb, nsmb07YY1INO80repair, nrmGasserrev, MolCell06CS, NatCellBiol07Misteli, Lecture Notes |
|
| Mon, 3/10/08 | (17) Chromatin abnormalities and
human disease I (Steffan) Matt, Rich, Kim, Hsiang, Wang review part 1, Wang review part 2, Shi review, Esteller review, Matt Janes, Lecture Notes |
|
| Thu, 3/13/08 | (18) Chromatin abnormalities and
human disease II (Thompson) Em: Steffan et al, Sir2 inhibitors and PD, Rett and chromatin 2007, Ataxin-7 and STAGA PLoS 2006 |
