Haoping Liu
Ph.D., Cornell University, 1991
University of California, Irvine
D250 Medical Sciences I
Irvine, CA 92697
Office: (949) 824-1137
h4liu@uci.edu
UCI Faculty Profile: Haoping Liu
Research Interests:
We use a combination of genetics, genomics and biochemistry in yeasts to study signaling pathways that control cellular development and morphogenesis. (1) We use the model yeast Saccharomyces cerevisiae to study mechanisms that maintain signaling specificity through MAP kinase pathways. (2) Our major focus is Candida albicans, the most prevalent fungal pathogen of humans. Its pathogenicity is dependent on the ability of C. albicans to undergo morphological transition (yeast-to-hypha) and phenotypic switching. We study molecular mechanisms that control these transitions. (3) We use protein microarrays to study host humoral responses to Candida infections. We are also studying killing mechanisms of antifungal peptides.
Our research is currently funded by NIH grants GM/AI55155 and AI061537.
Selected Publications:
Liu, H. and Bretscher, A., Purification of tropomyosin from Saccharomyces cerevisiaeand identificationof related proteins in Schizosaccharomyces and Physarum. Proc. Natl. Acad. Sci. USA 86, 90-93, 1989.
Liu, H., and Bretscher, A., Disruption of the single tropomyosin gene in yeast results in the disappearance of actin cables from the cytoskeleton. Cell 57, 233-242, 1989.
Liu, H., and Bretscher, A., Characterization of TPM1 disrupted yeast cells indicates an involvement of tropomyosin in directed vesicular transport. J. Cell Biol. 118, 285-299, 1992.
Liu, H., Krizek, J., and Bretscher, A., Construction of a GAL1 -regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast. Genetics 132, 665-673, 1992.
Liu, H., Styles, A. C., Fink, G. R., Elements of the yeast pheromone response pathway required for filamentous growth of diploids. Science 262, 1741-1744, 1993.
Liu, H., Köhler, J., Fink, G. R., Suppression of hyphal formation in Candida albicans by mutations of a STE12 homolog. Science 266, 1723-1726, 1994.
Liu, H., Styles, A. C., Fink, G. R., Saccharomyces cerevisiae S288c has a mutation in FLO8,a gene required for filamentous growth. Genetics144, 967-978, 1996.
Loeb, J., Sepulveda-Becerra, M., Hazen, I, Liu, H., A G1 cyclin is necessary for maintenance of filamentous growth in Candida albicans. Mol Cel Biol. 19, 4019-4027, 1999. available online
Loeb, J., Kerentseva T. A., Pan T., Sepulveda-Becerra, M., Liu, H., Saccharomyces cerevisiae G1 cyclins are differentially involved in haploid invasive and diploid pseudohyphal growth independent of the filamentation mitogen-activated protein kinase pathway. Genetics 153, 1535-1546, 1999. available online
Chen, J., Zhou, S., Wang, Q., Pan, T., Liu, H., Crk1, a novel Cdc2-related protein kinase, is required for hyphal development and virulence in Candida albicans. Mol Cel Biol. 20, 8696-8708, 2000. available online
Hazan, I., Sepulveda-Becerra, M., Liu, H., Cell polarity during hyphal morphogenesis is independent of cell cycle in Candida albicans. Mol Biol Cell. 13, 134-145, 2002. (E-publication on Dec. 7, 2001). available online
Lane, S., Zhou, S., Pan, T., Dai, Q., Liu, H. , Cph2, a member of bHLH proteins, is a regulator of hyphal development in Candida albicans . Mol Cel Biol. 21, 6418-6428, 2001. available online
Lane, S., Birse, C., Zhou, S., Matson, R., Liu, H., DNA Array Studies Demonstrate Convergent Regulation of Virulence Factors by Cph1, Cph2, and Efg1 in Candida albicans. J. Biol Chem. 276, 48988-48996. 2001 (E-publication on Oct. 10, 2001). available online
Liu, H., Transcriptional Regulation of Dimorphism in Candida albicans, Current Opinions in Microbiology 4, 728-735, 2001. available online
Liu, H. Construction of yeast libraries. Guide to Yeast Genetics and Molecular Biology Method in Enzymology, 350, 72-86. 2002.
Chen, JY., Chen, J., Lane, S., Liu, H., A Conserved Mitogen-Activated Protein Kinase Pathway Is Required For Mating in Candida albicans, Mol. Microbiology 2002. available online
Liu, H., Co-regulation of pathogenesis with dimorphism and phenotypic switching in Candida albicans, a commensal and a pathogen, Int. J. Med. Microbiol. 292, 2002.
Hazan, I., Liu, H., Hyphal tip-associated localization of Cdc42 is F-actin dependent in Candida albicans, Euk Cell 1(6), 856-864, 2002. available online
Hazan, I., Liu, H., Regulation of Morphogenesis by Conserved Developmental Pathways in Pathogenic Fungi. Pathogenic Fungi: Fungal Dimorphism and Pathogenicity, 2004.
Ni J, Gao Y, Liu H, Chen J., Candida albicans Cdc37 interacts with the Crk1 kinase and is required for Crk1 production. FEBS Lett.12; 561(1-3):223-30, 2004. available online
Chou, S, Huang, L, Liu, H, Fus3-regulated Tec1 degradation through SCFCdc4 determines MAPK signaling specificity during mating in yeast, Cell.119, 981-990, 2004. available online
Fang Cao, Shelley Lane, Prashna A Pala Raniga, Yang Lu, Zhou Zhou, Karalyn Ramon, Jiangye Chen and Haoping Liu, The Flo8 transcription factor is essential for hyphal development and virulence in Candida albicans, Mol Biol Cell. 2006 Jan;17(1):295-307. available online
Mao, X., Cao, F., Nie, X., Liu, H., Chen, J., The Swi/Snif chromatin remodeling complex is essential for hyphal development in Candida albicans, FEBS Letters 580 (2006) 2615-2622. available online
Chou, S., Lane, S., Liu, H., Regulation of mating and filamentation genes by two distinct Ste12 complexes in Saccharomyces cerevisiae. Mol Cell Biol. 2006 Jul;26(13):4794-805. available online
Huang G, Wang H, Chou S, Nie X, Chen J, Liu H. Bistable expression of WOR1, a master regulator of white-opaque switching in Candida albicans. Proc Natl Acad Sci USA. 2006 Aug 22; 103(34);12813-8. available online
Wang, A., Lane, S., Tian Z., Sharon, A., Hazan, I., Liu, H., Temporal and spatial control of HGC1 expression results in Hgc1 localization to the apical cell of hyphae in Candida albicans. Eukaryot Cell. 2007 Feb;6(2):253-61. available online
